Investigation of surface-liquid-interaction-Possibilities and pitfalls with Laser scanning microscopy as method

SpeakerDoc. Frank Schellenberger 

Time24th October 20182:30pm-4:30pmCommunication Building 818

Location818 Conference Room, Communication Building

TitleInvestigation of surface-liquid-interaction-Possibilities and pitfalls with Laser scanning microscopy as method


AbstractAbstract:Laser scanning confocal microscopy (LSCM) is widely used in biological and medical research for the three-dimensional imaging of cells and sub-cellular structures. However, the capabilities of LSCM have been hardly exploited in imaging wetting and interfacial phenomena. Wetting plays an important role in a wide variety of technological, biological, and environmental processes. Currently, a lot of research is devoted to controlling wetting via super-hydrophilic or super-hydrophobic surfaces. Understanding the interplay between microscopic structure and macroscopic wetting properties is necessary for the optimization of these surfaces. Inverted LSCM are ideally suited to analyse is wetting of structured surfaces because it allows to observe the important solid-liquid interface at high resolution.LSCM can operate in two modes; fluorescence, which requires labelling of the structures to be imaged with fluorescent dyes, and reflection mode, where the interfaces can be observed directly. The general operating principles of LSCM and an overview with best practise examples will be presented including our last work about capillary bridges and forces simultaneously investigated with AFM (Atomic Force Microscopy) and LSCM.

Biography:Frank Schellenberger studied Physics at the University of Mainz. Already as an undergraduate he started to work on image evaluation of wetting phaenomena. For his diploma thesis 2013 he built a home-made inverted Laser Scanning Confocal Microscope (LSCM) , which is combined with an Atomic Force Microscope (AFM) to image the shape of the meniscus between the particle and the liquid film and to measure the corresponding capillary force simultaneously. This work he did with Periklis Papadopoulos in the group of Doris Vollmer at the Max Planck Institute for Polymer Research (MPIP) in Mainz. He continued his work in his PhD thesis in the group of Hans-Jürgen Butt at the MPIP. Here he used and developed laser scanning confocal microscopes for the investigation of liquid contact lines. Preparation of structured super-hydrophocic surfaces and lubricant-infused surfaces for their characterisation. He received his doctoral degree from Mainz University 2018. Today, he works at the MPIP as a PostDoc. His actual research focuses on finding the limits of super resolution microscopy in material science in cooperation with the group of Christoph Cremer at the Institute of Molecular Biology (IMB).